RNAi-mediated MLL/AF4 knock-down time course

MLL/AF4 fusion transcript knock-down time course in the MLL/AF4-positive B-cell precursor ALL cell line SEM using MLL/AF4 fusion site-specific siRNAs (siMLL/AF4). The aim was to identify genes responsive to MLL/AF4 expression modulation. The B-cell precursor ALL cell line SEM cell line was sequentially transfected with either siRNAs against MLL/AF4 (siMLL/AF4), or control siRNA (siCtrl) at two day intervals for up to 3 times, representing a siRNA treatment time course of 6 days. For internal QC, mock-electroporated (MOCK) samples, representing pulse controls, were also included at the first and second electroporation. Material was harvested for analyses immediately prior to the subsequent electroporation and at the final time point, two days after the 3rd electroporation, corresponding to a sustained MLL/AF4 knock-down period of 2 (TP1), 4 (TP2) and 6 days (TP3), respectively. Differential expression analysis were performed for siCtrl and siMLL/AF4 samples at TP1, TP2 and TP3.