Binding affinities of [3H]SQ29,548 in HEK293 cells transiently expressing the WT or mutant human TP receptors.

Binding affinities and capacities were obtained by simultaneous analysis of at least 3 independent mixed-type experiments each performed in duplicates, analyzed with GraphPad Prism implemented with the LIGAND model (see Data and statistical analysis).aIn cotransfection experiments, TP and Gαq plasmids were added in a 1∶3 (3x) and 1∶5 (5x) ratio (see methods for details).bParameters refer to E129/240V double mutant before SQ29,548 rescue.