Global alternative RNA splicing analysis of Epstein-Barr Virus EBNA1-regulated cells

Purpose: To identify the effect of EBV EBNA1 on cellular mRNA alternative splicings Methods: HEK293 cells were transfected with either Flag-EBNA1 vector or empty vector (NC) for 48 hours. Then the cell RNA was sequenced by a PromethION platform (P48-seq, the third generation sequencing). Results: Log-fold changes of up- or down-regulated mRNAs between the control and experiment group were selected with a significance threshold of p<0.05. Conclusions: Our study describes the mRNA alternative splicing changes induced by EBV-EBNA1 in HEK293 cells Overall design: HEK293 cells were transfected with either Flag-EBNA1 vector or empty vector (NC) for 48 hours, and cellular RNA was used to sequence by Nanopore PromethION 48 (the third generation sequencing) to show the mRNA splicing profile changes induced by EBV-EBNA1 transfection.