Supplemental: Two photon microscopy allows for rapid processing of large Mohs specimens

Mendeley Supplemental Figure 1: A region of a two-photon image of a negative BCC Mohs surgery sample stained with AO/SYBR Green(nuclei) and SR101 (protein) (A) along with a zoomed region containing a hair follicle (B). The corresponding H&E stained FSA section for the specimen (C) along with a zoomed region (D) containing the same hair follicle shown above (B). Here we see great coregistration between the two zooms of the hair follicle between the MPM and H&E images. Mendeley Supplemental Figure 2: A region of a two-photon image of a negative BCC Mohs surgery sample stained with AO/SYBR Green(nuclei) and SR101 (protein) (A) along with a zoomed region containing sebaceous glands (B). The corresponding H&E stained FSA section for the specimen (C) along with a zoomed region (D) containing the same sebaceous glands shown above (B). Here we see great coregistration between the two zooms of the sebaceous gland between the MPM and H&E images.