Application of next generation semiconductor based sequencing of a plant DNA barcoding region to detect the botanical composition and geographic origin of monofloral, polyfloral and honeydew honey

In this study, we tested the Ion Torrent next generation sequencing technology to detect the botanical contribution of honeys of different origin and main botanical composition by analyzing DNA sequencing data obtained from a chloroplast trnL gene region using a customized bioinformatic pipeline that was designed including a reference dataset of more than 150,000 sequences.