Mitochondrial function measurement across 4 different rat liver Tru-cut ®biopsy sizes (18 samples; 6 rats).
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Oxidative phosphorylation is the measure of complex I State 3 respiration after the addition of complex I supporting substrates (10 mM glutamate, 5 mM malate, 10 mM pyruvate) and 1.25 mM ADP, while electron transport chain is the measure of the maximal electron transport chain capacity after the addition of the chemical uncoupler (carbonylcyanide p-trifluoromethoxy-phenylhydrazone, 1.5 µM FCCP). Respiratory control ratio is a measure of coupling efficiencies (rate in presence of ADP/rate in presence of complex I supporting substrates).a, Mean CV from each of the 6 animals with calculated SD; b, Combined CV of 18 replicates; Unpaired t-test: c, Pd, P
创建时间：
2013-10-29
