Transcriptome profiling of control and Zmynd8-cKO mouse cardiomyocyte

Appropriate gene expression within cardiomyocytes is coordinated by chromatin factors and is essential for heart function. We investigated the role of the chromatin reader ZMYND8 in the mouse heart using null and conditional knockouts (Zmynd8-cKO). While full-length Zmynd8 is not required for cardiomyocyte development, Zmynd8-cKO mice develop cardiomegaly, decreased cardiac function, and premature death compared to controls. Transcriptome analysis of Zmynd8-cKO cardiomyocytes reveals illegitimate expression of transcripts and proteins normally limited to skeletal muscle and integration of TNNI2 skeletal troponin into cardiac sarcomeres of mutant mice. We conclude that ZMYND8 is necessary to maintain appropriate cardiomyocyte homeostasis and gene expression. Overall design: Transcriptome profiles of 2 replicates of isolated cardiomyocytes from Myh6-CreTg/0 female mice, 2 replicates of isolated cardiomyocytes from Myh6-CreTg/0; Zmynd8fl/fl female mice, and 2 replicates of isolated cardiomyocytes from Myh6-CreTg/0; Zmynd8fl/fl male mice