Improvement of Peptide Separation for Exploring the Missing Proteins Localized on Membranes

How to separate hydrophobic peptides and to prevent loss of such peptides, nevertheless, have not been drawn enough attention (19). As a membrane protein possesses higher hydrophobicity, especially for one with multiple bilayer-exposed domains, efficient detection of hydrophobic peptides becomes a critical issue for its identification. It is generally recognized that MPs have two characteristics, relatively shorter sequences of amino acids and higher hydrophobicity.