RNA-seq analysis of human cells overexpressing a novel short XIST transcript isoform

We identified a novel short XIST isoform (sXIST) that is expressed in both male and female peripheral glia specifically. To study its functions, we overexpressed a fragment of it in the human HEK293T cell line and performed RNA-seq analysis of the sXIST overexpressing cells and GFP controls. We found that the up-regulated genes were enriched in pathways related to cilia functions and many share miRNA binding sites with the sXIST. Overall design: HEK-293T cells were transfected with a sXIST construct or GFP. mRNA from the virus-infected cells was iisolated and analyzed by RNA-seq.