Cisplatin-DNA Adduct Repair of Transcribed Genes is Controlled by Two Circadian Programs in Mouse Tissues

We tested the impact of clock time on excision repair of cisplatin-induced DNA damage at single nucleotide resolution across the genome in mouse liver and kidney. We found that genome repair is controlled by two circadian programs. Overall design: we perform XR-seq in mouse kidney and liver at different Zeitgeber time. After we injected the mouse with cisplatin 10mg / kg 2 hours, we sacrificed the mouse at indicated time and took the kidneys and liver. then lysed tissue and did TFIIH IP and cisplatin IP for construct the XR-seq library and sequence.