Glutathione peroxidase and thioredoxin reductase activities in turkey tissues.

1GPX1 and GPX4 activities determined on 15,000 x g supernatants of 10% homogenates, as described by Sunde and Hadley [4], and prepared from the same frozen tissues used for total RNA analysis. Gpx4 activity assayed using 78 μM PCOOH. GPX1 activity was assayed using 120 μM H2O2, and then corrected for GPX4-dependent activity, to yield the GPX1 activities shown in the table. Activity values are expressed per gram protein.
2TXNRD activities determined on dialyzed 15,000 x g supernatants of 10% homogenates using gold-thioglucose inhibition of 5,5’-dithiobis (2-nitrobenzoic acid) reduction, as described by Barnes et al. [5]. Activity values are expressed per gram protein.
Glutathione peroxidase and thioredoxin reductase activities in turkey tissues.