Transcriptomic data of BT549 triple negative breast cancer cells treated with 20 μM NU7441,a DNA-dependent kinase inhibitor

DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is a multifunctional serine-threonine protein kinase that plays pleiotropic roles in cancer.NU7441 is a specific DNA-PKcs inhibitor.We investigated the effect of the DNA-dependent kinase (DNA-PK) inhibitor, NU7441, on the transcriptome of BT549 triple negative breast cancer (TNBC) cells.Total RNA extracted from NU7441 or vehicle-treated cells was processed for preparation of sequencing libraries . Assessment of read quality was performed using fastqc tool. Trimming and filtering low-quality reads were performed using fastp. Reads were aligned by hisat2. SAM files were converted to BAM files using Samtools. Mapped reads were quantified using featureCounts.The RNA sequencing results were also subjected to gene differential expression analysis, Gene Ontology (GO) analysis and KEGG pathway analysis. After NU7441 treatment, total number of 2045 differential genes were selected according to |log2(FoldChange)| >= 1 & padj<= 0.05, among which 1365 genes were down-regulated and 680 genes were up-regulated. The differential genes in pattern recognition receptors (PRRs) immune responses signals, including NOD-like receptor signaling, Toll-like receptor signaling, RIG-I-like receptor signaling and Cytosolic DNA-sensing pathways were noted in this paper .