TABLE 3 in Genetic analysis of a Mediterranean Red-Listed species Brachytrupes megacephalus (Lefèbvre, 1827) (Orthoptera, Gryllidae)

TABLE 3. PCR programme conditions used for each primer pair in this study. Primer NosInitial denaturationAmplificationFinal extensionReferenceCyclesDenaturationAnnealingElongation1 and 24 min at 94°C4030 s at 94°C30 s at 47°C40 s at 72°C7min at 72°CKamphambati 1995 as cited in Chintauan-Marquier et al. 20163 and 44 min at 94°C5050 s at 94°C50 s at 52°C50 s at 72°C7 min at 72°CGiribet et al. 1999 as cited in Chintauan-Marquier et al. 20165 and 64 min at 94°C4550 s at 94°C40 s at 58°C40 s at 72°C7 min at 72°CSvenson & Whiting 2004 as cited in Chintauan-Marquier et al. 20167 and 84 min at 94°C4040 s at 94°C40 s at 50°C60 s at 72°C7 min at 72°CWhiting 2002 as cited in Chintauan-Marquier et al. 20169 and 104 min at 94°C4035 s at 94°C35 s at 57°C50 s at 72°C7 min at 72°CKamphambati 1995 as cited in Chintauan-Marquier et al. 201611 and 124 min at 95°C3540 s at 94°C40 s at 50°C60 s at 72°C8 min at 72°CPradit et al. 202213 and 144 min at 95°C3540 s at 94°C40 s at 48°C60 s at 72°C8 min at 72°CPradit et al. 2022