SKP1A-mediated degradation of PRC2 triggers activation of Polycomb-silenced genes

Polycomb group (PcG) proteins are evolutionarily conserved repressors of developmental genes. Paradoxically, the same PcG proteins also play roles in gene activation via mechanisms that are not yet fully understood. Here, to reveal the function of S-phase kinase-associated protein 1A (SKP1A), an essential factor of SCF (SKP1A/CULLIN1/F-box) ubiquitin ligases, polyubiquitinated proteins by SKP1A were prepared by pull-down assays and characterized by LC-MS/MS