Anti-aging Metabolite-Based Polymeric Microparticles for Intracellular Drug Delivery and Bone Regeneration. Anti-aging Metabolite-Based Polymeric Microparticles for Intracellular Drug Delivery and Bone Regeneration

In this study, we compared the gene expression profiles of mouse MC3T3-E1 cells treated with PAKG MPs to those of the control group. Overall design: mouse MC3T3-E1 cells were treated with PAKG–8diol–PEG(S) MPs (30 μg mL−1) for 2 days before cell harvested for total RNAs isolation. The cells without MPs treatment and cultured in the same OC medium were served as control group. Poly(A) RNA-sequencing library construction and sequencing, transcripts assembly, and different expression analysis were performed.