Expression profiling of death-related genes in ZIKV-infected SH-SY5Y cells

Analysis of death triggering pathways activated by experimental Zika virus infection in SH-SY5Y cells by expression profiling of 42 genes related to survival, anti-apoptotic and proapoptotic responses, and death receptors pathway, and 06 endogenous control genes. Commercially available neuroblastoma SH-SY5Y cells were grown in vitro, experimentally infected with Zika virus (MOI 0.5), and MOCK- and ZIKV-infected cells were harvested at 1 and 2 days post-infection. We used TaqMan ™ Array Human Apoptosis via Death Receptors (Thermo Fisher Scientific, MA, USA) to quantitate gene expression during ZIKV infection in SH-SY5Y cells. qPCR gene expression profiling. Two replicate samples of each timepoint post-infection (1 and 2 days post-infection) were infected separately as indicated in the summary. We used two replicate samples of mock-infected cells as control samples. Equal amounts of cDNA of each timepoint was added to each real time PCR reaction.