Single cell transcriptome analysis of E15.5 and E17.5 mouse coronal sutures - all clusters

E15.5 and E17.5 coronal sutures were dissected from calvaria and individual cells isolated for scRNAseq. Transcriptome libraries for single cells were captured using 10X genomics Chromium Single Cell 3’ Library and Gel Bead Kit v2 following manufacturer’s guidelines. Sequencing for E15.5 coronal sutures was performed with Illumina’s HiSeq 3000/4000 PE Cluster Kit at the Children’s Hospital Los Angeles’ Molecular Genomics Core, and for E17.5 cells, PE sequencing was run on an Illumina HiSeq 4000 at the Oxford Genomics Centre Wellcome Centre for Human Genetics, Oxford achieving an average of ~150,000 mean reads per cell for E15.5 and ~92,000 mean reads per cell for E17.5.