Data and code from: Effects of ploidy on relationship between outbreeding response and fitness in a plant selfing species

Outbreeding response, the phenotypic differences observed between selfed parental lines and their outcrossed offspring, can influence the evolution of selfing strategies. However, such an effect remains poorly understood in non-crop species. We explored the phenotypic outbreeding response variation across ploidy levels in Erysimum incanum, a predominantly selfing plant complex with diploid, tetraploid, and hexaploid populations distributed across the Iberian Peninsula and Morocco. We performed controlled within-population crosses to generate offspring with varying heterozygosity levels across ploidy types. We quantified individual, flower, and reproductive traits, and we estimated fitness components, and assessed trait modularity and phenotypic integration to see how heterozygosity affects trait coordination. Tetraploids showed the strongest and most consistently positive outbreeding responses, particularly in gamete production. Trait-specific outbreeding responses were positively assoc..., , # Data and code from: Effects of ploidy on relationship between outbreeding response and fitness in a plant selfing species

Dataset DOI: [10.5061/dryad.ngf1vhj7w](https://doi.org/10.5061/dryad.ngf1vhj7w)

## Description of the data and file structure

Here we provide the dataset generated during the performance of experiments done for the article \"Effects of ploidy on relationship between outbreeding response and fitness in a plant selfing species\" and a HTML file with a detailed development of written code to analyze them using R.

### Files and variables

#### File: Scripts_OR_EcolEvol.html

**Description:** HTML file containing detailed explanations and code to analyse data.

#### File: Data_OutbreedingResponse_2025.csv

**Description:** CSV separated by semi-colon with comma as decimal mark containing all phenotypic data collected in the experiment and used for statistical analysis. NAs contained in the file indicate there was not a given measurement for that individual due to loss...,