Gene expression profiles in gastric cancer cells with TM4SF1-AS1 knockdown

To clarify the function of a long noncoding RNA TM4SF1-AS1 in gastric cancer (GC), we carried out a gene expression microarray analysis using GC cell lines HSC45 transfected with a siRNA targeting TM4SF1-AS1 or a control siRNA. Gene ontology analysis revealed that genes associated with interferon signaling and immune response were significantly enriched among downregulated genes. HSC45 cells were transfected with a siRNA targeting TM4SF1-AS1 or a control siRNA. Ninety-six hours after transfection, total RNA was extracted and gene expression profiles were analyzed.