RNA-seq analysis of parent, RPL22L1 knockout and RPL22 reconstituted HCT116 cells.

Ribosome biosynthesis is essential for cancer growth and proliferation. This dependency is therapeutically exploitable by blocking the rate-limiting step, RNA polymerase I (Pol I) transcription. We discovered that RPL22 and RPL22L1 were markers for sensitivity to Pol I inhibition. We discovered that their genetic manipulation prominently altered the expression and splicing of a multitude of mRNAs. Overall design: We generated RPL22L1 knockout and RPL22-reconstituted HCT116 cells. RNA sequencing (RNA-seq) was performed with three replicates for each group. Differential expression and alternative splicing were analyzed.