Birefringent gut granules in embryos.

All strains were grown at 22°C, unless noted. Three-fold and later stage embryos were analyzed using polarization microscopy and scored for the presence and localization of birefringent material in the intestine. n = number of embryos scored.1Embryos were derived from glo-2(tm592) adults grown at 15°C. The phenotype of wild type and glo-2(zu455) were not altered by growth at 15°C (data not shown).2The progeny of glo-2(−)/+ animals were scored. ¼ of the progeny were expected to be glo-2(−)/glo-2(−).3Reciprocal crosses of glo-2(tm592) males with glo-2(zu455) hermaphrodites and glo-2(zu455) males with glo-2(tm592) hermaphrodites were performed to generate glo-2(tm592)/glo-2(zu455) adults. Pretzel-stage progeny of these animals were scored and were predicted to be ½ glo-2(tm592)/glo-2(zu455), ¼ glo-2(tm592)/glo-2(tm592), and ¼ glo-2(zu455)/glo-2(zu455).