Response to SARS-CoV-2 infection in cornea, limbus and sclera from human donors

Here we report the transcriptional response to SARS-CoV-2 in cornea, limbus and sclera from human cadaver donor tissue. All three tissues are characterized by an NFkB-mediated chemokine response to viral infection as well as type-I interferon stimulated genes. Cornea, limbus and sclera was isolated from human donor tissue and passaged in tissue culture. Cells were infected with SARS-CoV-2 for 24 hours in triplicate and the host response was compared to mock-infected samples.