The responses of WT root and sgn3 myb36 root inoculated with CHA0 for 0h, 3h, 6h and 10h

The casparian strip serves as a crucial diffusion barrier in the endodermis, playing a vital role in controlling the flow of substances between the root and its external environment. Here, we observed that when the casparian strip is absent, bacteria can accumulate abundantly on the roots. To investigate the impact of this bacterial accumulation on the root indued by casparian strip deficiency , we inoculated Col-0 and sgn3 myb36 roots with CHA0 in a hydroponic system. Ultimately, we found that the bacterial accumulation on the roots, resulting from the absence of the Casparian strip, strongly induces the immune response of the roots. This indicates that the casparian strip plays an important role in regulating the interaction between the root and microorganisms. To investigate the impact of this bacterial accumulation on the root indued by casparian strip deficiency, we inoculated Col-0 and sgn3 myb36 root with CHA0 for 0h, 3h, 6h and 10h in a hydroponic system. We then performed gene expression analysis using rna seq data from Col-0 and sgn3 myb36 root treated with CHA0 at 4 time points. For DEG analysis, data from another three time points was compared with 0h.