Comparisons between methods.

Macroinvertebrate families found by visual observation (visu) and through next-generation sequencing employing the 18S and COI genes, with Assignment criteria #4 for the latter, at each sampling point (marked with “X”). Proportion of false negatives considering all the sampling sites. Number of positives: the number of times each family was detected through sampling points with each methodology (COI,18S and visual); employed to calculate Fisher´s exact test.