Text S1 - Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice

Text S1 describes the method analyzing the activity of Tre against LTR sites of different HIV-1 strains and provides supporting figures demonstrating the Tat responsiveness of LV constructs, data plots of Tre analysis in HeLa-smurf cells, mapping of HIV integrations sites in HeLa-smurf cells, cellular growth curves upon Tre expression, detailed array-CGH analysis of the human chromosomes, Tre activity testing on different HIV-1 isolates, analysis of HIV-1 coreceptor expression in LV-transduced CD4+ T cells, and FACS analyses of single cell suspensions derived from various organs of Tre-transduced and HIV-infected mice. Finally, the sequences of bar-coded fusion primers used for pyrosequencing are provided. (PDF)