CRISPR/Cas9-induced modification of VRN-A1 gene of bread wheat.. Triticum aestivum cultivar:Chinese spring

We attempted to expand the types of VRN-A1 alleles by the CRISPR/Cas9 targeted modification of promoter sequence. New mutations stably inherited in next progenies and transgene-free homozygous plants carrying novel VRN-A1 variants were generated. CRISPR/Cas9-mediated deletion of vrn-A1 promoter shortened the time of head emergence up to 2-4 days.