Transcriptomic changes in SJRHB015721_X1 human FN-RMS following DBX1 knock-down. Transcriptomic changes in SJRHB015721_X1 human FN-RMS following DBX1 knock-down

DBX1 is a transcriptional repressor whose role in human cancer is poorly understood. DBX1 is highly expressed in FN-RMS. Here, we depleted DBX1 expression with 2 shRNAs targeting DBX1 in a human FN-RMS cell line we derived from a PDX - SJRHB015721_X1. We determined the transcriptional changes using a Clariom S human microarray. Overall design: One of two shRNAs targeting DBX1 (or a scramble control) were transduced into SJRHB015721_X1 cells. RNA was harvested 72 hours later using Qiagen's miRNeasy RNA isoloation kit.