Probing endogenous protein localization and function in vivo remains challengingdue to laborious gene targeting and monofunctional alleles. Here, we develop amultifunctional and adaptable toolkit base
Base editors, mainly including cytidine base editors(CBE) and adenine base editors(ABE), have been widely used to modify single-base in genomic DNA. The efficiency of A-to-G towards the PAM for ABE an
Although significant efforts have been directed at developing efficient techniques for rare and super rare genome cutting, only limited success has been achieved. Here we propose a new approach to sol
