Gene expression comparison between yeast histone H2B WT and H2B R95A mutant without and with rapamycin treatment.

This microarray data is the Affymetrix array of RNA expressed by Wild Type and Mutant strains yeast that is untreated and treated with rapamycin. In yeast, rapamycin inhibits the TORC1 kinase signaling pathway causing rapid alteration in gene expression and ultimately cell cycle arrest in G1 and this study provides a new insight whereby rapamycin signaling via the Torc1 kinase may exploit the pheromone pathway. Wild Type and Mutant Yeast cultures were either untreated or treated with rapamycin and extracted using the hot phenol extraction protocol. Microarray analysis was performed using Affymetrix GeneChip® and the data was normalized using robust multi-array average (RMA) with FlexArray tool.