Role of Caveolin 1 in metabolic programming of fetal brain (bulk RNA-seq data)

Caveolae are plasma membrane invaginations found in most cells of mammals. Caveolin-1 (Cav1) encodes a major protein of the lipid rafts of these membrane structures. Cav1-null mice, though viable, show various phenotypic defects. At an early adult age, these mice show brain aging that resemble brain of one and half year old wildtype mice, and exhibit symptoms that are hallmarks of Alzheimer's disease. It is not known if the ablation of Cav1 in these mice impacts the brain at the fetal stage that then influences brain function later in life. RNA-seq was performed to profile gene expression of fetal brain (gestation day 15) and aging brain (week 70) of Cav1 knockout mice. The data was comapred with genes expression data of fetal brain (gestation day 15) and aging brain (week 70) of wildtype mice from our earlier study. RNA-seq was performed with fetal brain (gestation day 15) of wild-type and Cav1 knockout mice. Aging brain (week 70) from Cav1 knockout mice was also sequenced and compared with RNA-seq data of WT mice generated from our earlier study.小窝（Caveolae）是存在于多数哺乳动物细胞中的质膜内陷结构。小窝蛋白1基因（Caveolin-1, 缩写为Cav1）可编码这类膜结构脂筏（lipid rafts）的核心蛋白。Cav1基因敲除（Cav1-null）小鼠虽可存活，但表现出多种表型缺陷。该类小鼠在成年早期即可出现脑衰老表型，其脑组织状态与1.5岁龄野生型小鼠相仿，同时表现出阿尔茨海默病（Alzheimer's disease）的标志性症状。目前尚不清楚该类小鼠中Cav1的敲除是否会在胎儿阶段影响脑组织，进而影响其成年后的脑功能。本研究通过RNA测序（RNA-seq）对Cav1基因敲除小鼠的胎脑（妊娠第15天）及70周龄衰老脑组织进行了基因表达谱分析，并将所得数据与本团队前期研究中野生型小鼠的对应组织基因表达数据进行比对。此外，本研究还对野生型及Cav1基因敲除小鼠的胎脑（妊娠第15天）开展了RNA测序，同时对70周龄Cav1基因敲除小鼠的衰老脑组织进行测序，并将其与本团队前期研究中野生型小鼠的RNA测序数据进行比对。