Gene expression changes induced by SMPD1 or GPR64 silencing in A673 Ewing Sarcoma cells. Gene expression changes induced by SMPD1 or GPR64 silencing in A673 Ewing Sarcoma cells

We used RNA-sequencing to compare the gene expression changes induced by SMPD1 silencing and GPR64 silnecing. Overall design: A673 cells were were transfected with either scrambled-siRNA or SMPD1-siRNA or GPR64-siRNA for 48 hours. Transfection was carried out using Lipofectamine™ RNAiMAX Transfection Reagent (Thermo Fisher) according to the manufacturer’s protocol. After 48 hours, cells were harvested and total RNA was extracted using TRIzol reagent (Invitrogen). For whole-genome transcriptome profiling, four libraries were generated from total RNA sequencing.