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Isolation and MS characterisation of recombinant human septin octameric complexes containing distinct SEPT9 isoforms

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NIAID Data Ecosystem2026-03-12 收录
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Native human septins form heterooctameric complexes containing different SEPT9 isoforms. To enable studies of SEPT9 function, we established a protocol for the isolation of recombinant human octamers harboring distinct SEPT9 isoforms. Mass spectrometry analysis is used to assess the purity, integrity and stoichiometry of septins in the purified complex.

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2021-07-27
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