The Na+-pumping mechanism driven by redox reactions in the NADH-quinone oxidoreductase from Vibrio cholerae relies on dynamic conformational changes

The cryo-EM maps have been deposited in the EMDB under accession codes, 64518 (WTred), 63872 (NqrC-T225Yox), 64059 (NqrC-T225Yred), 64060 (NqrB-T236Yox), 64061 (NqrB-T236Yred), 64063 (WTred/ -Na+, “up” state), 64064 (WTred/ -Na+, “middle” state), 64065 (WTred/ -Na+, “down” state), 64062 (WTred + KR), 64069 (WTred + AD-42), 64066 (NqrB-G141Ared + KR, “stable” state), and 64068 (NqrB-G141Ared + KR, “shifted” state). The atomic models have been deposited in the Protein Data Bank under accession codes, 9UUU (WTred), 9U5G (NqrC-T225Yox), 9UD2 (NqrC-T225Yred), 9UD3 (NqrB-T236Yox), 9UD4 (NqrB-T236Yred), 9UD6 (WTred/ -Na+, “up” state), 9UD8 (WTred/ -Na+, “middle” state), 9UD9 (WTred/ -Na+, “down” state), 9UD5 (WTred + KR), 9UDG (WTred + AD-42), 9UDA (NqrB-G141Ared + KR, “stable” state), and 9UDF (NqrB-G141Ared + KR, “shifted” state).