Mu opioid receptor (Oprm1) knockdown in the medial preoptic area (MPOA) on patterns of gene expression in the MPOA of adolescent male rats

We investigate effects of Oprm1 downregulation in the MPOA on gene expression profiles in adolescent male rats using RNA-Seq. We conducted differential gene expression analysis of RNA-Seq data using the EdgeR Bioconductor Package. We evaluated differential gene expression between Oprm1 knockdown and control groups and found 349 genes were differentially expressed, with some genes being involved in neuronal signaling exhibited altered expression due to Oprm1 knockdown. Overall design: Examination of patterns of gene expression after Oprm1 knockdown in adolescent male rats