Single-cell RNAseq of Neurog1-Neurog2-DsRed transduced DRG cells

Cultured adult mouse dorsal root ganglia (DRG) cells exhibit glial sensory progenitor properties in vitro. Therefore, they might be a good starter cell for reprogramming into sensory neurons. Here, we infected them with the retroviral vector Neurog2-Neurog1-DsRed to induce sensory neuron development and analyzed by scRNAseq at 14 days post infection whether infected cells show properties of sensory neurons such as nociceptors. After 10x Genomics, data analysis of 4,549 individual cells indicated the generation of neurons, but at an immature cell state. Overall design: Cells from dorsal root ganglia culture of adult mice were infected with Neurog2-Neurog1-DsRed, DsRed-positive cells were isolated with Fluorescence-activated cell sorting (FACS) at 14 days post infection and analyzed using scRNAseq.