TDP-43 modulates translation of specific mRNAs linked to neurodegenerative disease

We generated total mRNA libraries and ribosome footprint libraries from motor neuron-like cells (MN1) and primary cortical neurons expressing GFPor pure wild type cells as control, human TDP-43 wild type and mutant proteins to identify translational targets of human TDP-43 mutant protein We used two replicates for MN1 cells and three replicates for primary neurons from which total mRNA and ribosome footprint libraries were generated. The libraries were multiplexed sequenced on an Illumina HiSeq2500 SR 50 base run.