Compatible solute production by sea ice bacteria

Two sea ice bacterial isolates (Polaribacter sp. ALD11 and Shewanella sp. ALD9) were grown in liquid media to either exponential or stationary phase, in low or high salinity media, and at low or high temperature. Cells were harvested by pelleting and immediate freezing in liquid nitrogen, followed by storage at -80C until shipment on dry ice. Subsamples were sent for metabolomic and transcriptomic analyses to evaluate the effects of environmental difference son gene expression and compatible solute content in these organisms.