Secreted protein relative abundance and activity from PhoA-Asaia protein fusion plasmids expressed in Asaia.

PhoA = alkaline phosphatase. Tsr = TonB-dependent siderophore receptor. Aap = amino acid permease. Tdr = TonB-dependent receptor plug. Gdh = glucose dehydrogenase. Ybp = YVTN beta-propeller repeat protein. Csy = cellulose synthase. Amu = aminomutase. Ptp = peptide transport permease. Both the ELISA and PNPP plates were repeated five times, and quantified using a plate reader. Signal intensity was read at 450 nm for the ELISA and at 400 nm for the PNPP assay. Although there was a similar amount of protein secreted by Tsr compared to Ybp, PhoA activity was more than five times as strong when secreted by Tsr. Each plate assay was performed 5 times and the means compared by an unpaired t-test using GraphPad Prism software, version 5.0.