Single Cell Sequencing Data of Mouse Spinal Cord with Dorsal Column Lesion and Astrocyte-Specific Ryk conditional Knockout

After the data quality control, a total of 18,203 captured cells were subjected to clustering analysis by leiden and visualized in lower dimension by uniform manifold approximation and projection (UMAP). The results showed that they could be clustered into 17 distinct cell clusters. According to the highest differentially expressed genes (DEGs) and canonical markers used in prior studies, we annotated these 17 clusters as 13 major cell types with distinct expression profiles): Astrocytes (ASC, containing 3 clusters: Astro_2, Astro_5, and Astro_7), microglia (MG, containing 2 clusters: Microglial_6 and Microglial_10), oligodendrocytes (containing 2 clusters: Oligo_4 and Oligo_8), endothelial cells (containing 1 cluster: Endothelial_1), pericytes (Pericytes_9), fibroblasts (Fibroblasts_14), neuronal cells (Neuronal_3), oligodendrocyte progenitor cells (OPC_11), Schwann cells (schwan cells_15), red blood cells (RBC_17), immune cells (Immuno_12), myeloid cells (myeloid Cells_16) We dissociated cells from the spinal cord tissue around the lesion core. Two biological repetitions of single cell suspensions were generated from the mice which uninjured, 1d, 7d, and 14d after SCI with or without Ryk cKO.