Anatomical Structures, Cell Types, plus Biomarkers (ASCT+B) table for Spleen, v1.5

Anatomical Structures, Cell Types, plus Biomarkers (ASCT+B) tables (https://humanatlas.io/asctb-tables) aim to capture<br>anatomical human body parts, the typology of cells, and biomarkers used to identify cell types. The tables are<br>authored and reviewed by an international team of experts. The spleen ASCT+B tables are derived from published<br>literature, public datasets, unpublished studies from table authors, and input provided by Dr. Birte Steiniger, an<br>early reviewer and leader in the field of spleen anatomy. The gene biomarkers are primarily derived from one landmark<br>publication in humans: [Madissoon et al. 2019] (https://doi.org/10.1186/s13059-019-1906-x). The anatomical structures<br>and cell types of the spleen were highly influenced by work from Dr. Steiniger including: [Steiniger 2015]<br>(https://doi.org/10.1111/imm.12469), [Steiniger et al. 2018] (https://doi.org/10.1038/s41598-018-34105-3), and<br>[Steiniger et al. 2007] (https://doi.org/10.1007/s00418-007-0320-8). Whenever possible, we used multiple sources to<br>confirm gene and protein biomarkers and united them across cell types. Cell phenotypes, especially for protein-based<br>assays, are highly granular with several markers listed at different expression levels. We anticipate further revision<br>and refinement from multimodal studies that pair sequencing and imaging studies from the same human sample. In total,<br>this table reports 37 anatomical structures, 60 cell types, and 194<br>biomarkers. <br> <br>**Bibliography:** <br><br>* Madissoon, E., Wilbrey-Clark, A., Miragaia, R. J., Saeb-Parsy, K., Mahbubani, K. T., Georgakopoulos, N., Harding, P., et al. 2019. “scRNA-Seq Assessment of the Human Lung, Spleen, and Esophagus Tissue Stability after Cold Preservation.” *Genome Biology* 21 (1): 1. https://doi.org/10.1186/s13059-019-1906-x. <br>* Steiniger, Birte S. 2015. “Human Spleen Microanatomy: Why Mice Do Not Suffice.” *Immunology* 145 (3): 334–346. https://doi.org/10.1111/imm.12469. <br>* Steiniger, B. S., Wilhelmi, V., Berthold, M., Guthe, M., and Lobachev, O. 2018. “Locating Human Splenic Capillary Sheaths in Virtual Reality.” *Scientific Reports* 8 (1): 15720. https://doi.org/10.1038/s41598-018-34105-3. <br>* Steiniger, Birte, Stachniss, Vitus, Schwarzbach, Hans, and Barth, Peter J. 2007. “Phenotypic Differences between Red Pulp Capillary and Sinusoidal Endothelia Help Localizing the Open Splenic Circulation in Humans.” *Histochemistry and Cell Biology* 128 (5): 391–398. https://doi.org/10.1007/s00418-007-0320-8.<br>* Börner, Katy, Sarah A. Teichmann, Ellen M. Quardokus, James C. Gee, Kristen Browne, David Osumi-Sutherland, Bruce W. Herr II, et al. 2021. “Anatomical Structures, Cell Types and Biomarkers of the Human Reference Atlas.” *Nature Cell Biology* 23 (11): 1117–28. https://doi.org/10.1038/s41556-021-00788-6."