Silencing of UHRF1 in PC3 cells induces changes in the miRNome

To understand the role of UHRF1 in prostate cancer, we have employed miRNAs microarray expression profiling as a discovery platform to identify miRNAs whose expression is regulated by UHRF1 and have a potential to regulate downstream effectors involved in prostate cancer. PC3 cells were silenced with siRNAs against UHRF1 or control siRNAs, in triplicate, as previously described (Babbio F, Pistore C, Curti L, Castiglioni I, Kunderfranco P, Brino L, Oudet P, Seiler R, Thalman GN, Roggero E, Sarti M, Pinton S, et al. The SRA protein UHRF1 promotes epigenetic crosstalks and is involved in prostate cancer progression. Oncogene 2012;31: 4878-87). RNA was collected 72 hours after transfection and knock-down of UHRF1 mRNA was validated by RT-PCR on each independent replicate sample.