Regulatory T cells in the spleen and lymph nodes following Flt3L treatment during mBSA arthritis.

A) Representative FACS plots illustrating effects of Flt3L-treatment on splenic and nodal Treg cells B) Relative increase (%) in Treg cells in the spleen and draining lymph nodes of Flt3L-treated mice compared to sham-treated controls during mBSA arthritis. C) Gene expression of the Treg associated genes, FoxP3 and Stat5b, in the spleen. D) Representative histogram of FoxP3 on CD4+FoxP3+ cells (Dashed line = isotype control, tinted grey = sham-treated and black = Flt3L-treated). E) Relative increase (%) in the intensity of FoxP3 expression (measured by gMFI) on individual Tregs in the spleen and lymph nodes of Flt3L-treated mice compared to sham-treated controls. Flt3L, day 10 n = 15 and day 28 n = 7; sham, day 10 n = 15 and day 28 n = 11. Relative increase was calculated as described in material and methods. Data are presented as mean ± SEM and statistical significance was assessed using unpaired t-test. *PPP