methyl-ATAC-seq measures DNA methylation at accessible chromatin. methyl-ATAC-seq measures DNA methylation at accessible chromatin

Here we describe methyl-ATAC-seq (mATAC-seq), which implements modifications to ATAC-seq, subjecting the output to BS-seq. Merging these assays into a single protocol identifies the locations of open chromatin, and reveals the DNA methylation state of the underlying DNA. Overall design: Examination of 2 different methods (Omni-ATAC-seq and mATAC-seq) on HCT116 and DKO (DNMT1 and DNMT3B double knockout) cells. Genome_build: hg19 Supplementary_files_format_and_content: bw for all samples, bedGraph and cov files also for mATAC-seq samples bw files for visualization of coverage generated using Deeptools bedGraph files for visualization of DNA methylation generated using Bismark cov files for analysis of DNA methylation generated using Bismark