Transcriptome profiling in primary human skeletal myotubes with MondoA knockdown. Transcriptome profiling in primary human skeletal myotubes with MondoA knockdown

It is well known that insulin resistance in the obese state is associated with accumulation of neutral lipid in extra-adipose tissues. This phenomenon is particularly well-documented in skeletal muscle. The mechanistic underpinnings of this linkage are not fully understood. To define new MondoA target gene and pathway in human myotubes, we performed whole genome RNA sequencing. Overall design: RNA seq was performed with total RNA isolated from differentiated primary human skeletal myotubes. These cells were transfected with siRNA of control or MondoA. Three biologic replicates for each condition were included.