Thermodynamic parameters of the thermal unfolding of the PSD95-PDZ3 domain and mutants in 50 mM potassium phosphate pH 7.5 obtained from the analysis of DSC experiments*.

*The error intervals were calculated as described in the text. The values of thermodynamic magnitudes for the U⇆In and I⇆In equilibriums were estimated for a Pref = 100 µM. The heat capacity functions obtained from the fittings, in kJ·K−1·mol−1 were: PDZ3: CpN = −9.23+(0.095*T); CpU = 2.91+(0.064*T); CpIn = −99.0+(0.347*T); D332G: CpN = −9.02+(0.093*T); CpU = 11.42+(0.045*T); CpIn = −113.0+(0.389*T); D332P: CpN = −12.8+(0.103*T); CpU = 6.69+(0.058*T); CpIn = −105.6+(0.364*T); E334L: CpN = −24.4+(0.143*T); CpU = −10.7+(0.104*T); CpIn = −38.4+(0.170*T); E334Q: CpN = −7.5+(0.085*T); CpU = 16.4+(0.029*T); CpIn = −85.5+(0.309*T); Δ10ct-PDZ3: CpN = −9.7+(0.096*T); CpU = 19.1+(0.117*T); CpIn = −30.4+(0.131*T); CpI = −31.9+(0.146*T); E401R: CpN = −13.6+(0.107*T); CpU = −34.4+(0.144*T); CpIn = −79.1+(0.284*T); CpI = −34.6+(0.138*T).aData taken from [21].bData taken from [24].