An integrated network pharmacology and RNA-seq approach for exploring the vascular protection of neferine on attenuating Ang II-induced blood pressure elevation

The goals of this study is to compare the differently expressed genes in abdominal aorta tissues of Control and Ang II with or without neferine treatment. The Ang II (n=12) were randomly divided into 2 groups: Ang II , and Ang II + neferine (1 mg/kg/day) groups (n=6 for each group). Control groups (n=5) were set as control. Mouse in Control and Ang II groups were intragastrically with double distilled water (dd H2O); while mice in Ang II + neferine (1 mg/kg/day) group were intragastrically with 1 mg/kg/day of neferine for 6 weeks. Then the abdominal aorta tissues were used to identify differentially expressed genes among different groups. RNA sequencing was performed with abdominal aorta of mice in 3 groups:Control(n=5), Ang II(n=6 ) , Ang II + neferine(1 mg/kg/day,n=6) treated to identify the differently expressed genes and related pathway.