Transcriptome analysis of bladder wall from patients with bladder outlet obstruction induced lower urinary tract dysfunction. Bladder_RNAseq

Introduction and Objectives: Bladder outlet obstruction (BOO) results in profound structural and functional changes in the detrusor, including hypertrophy and bladder decompensation (fibrosis). Previously we determined miRNA profiles characteristic of defined states of BOO in human patients and used bioinformatic prediction of their target genes to identify the regulated signalling pathways. Here we aim to do the mRNA transcriptome analysis of the same samples and correlate microRNA and mRNA regulation patterns in order to validate functional ontology. Materials and Methods: Controls, BOO with and without detrusor overactivity (DO and BO groups, respectively) and underactive bladders (UA group) were defined by urodynamics in human subjects. Bladder dome biopsies were collected and RNA isolated using mirVANA kit for miRNA. mRNA sequencing (mRNA-seq) method was used for gene expression profiling of BOO stages (4 groups, n = 6 per group). The Enrichment analysis using GeneGo MetaCore software was applied to the significantly altered mRNAs. Functional analysis of a network identified the most significant biological functions of the genes in the network.