Single-cell RNA sequencing of the testis of drive and standard Teleopsis dalmanni males

Sex-linked meiotic drivers limit the inheritance of the alternate sex chromosome in the heterogametic sex, subsequently skewing the ratio of males to females in the offspring. They consequently have large impacts on genome evolution, adaptation, and the emergence and maintenance of sexually selected traits. Detecting the molecular basis of meiotic drive is complex. It is affected by both the common occurrence of inversions housing a driver as well as the transcriptomic and cell type heterogeneity of the gonads. Herein, we utilise DNA-Seq to investigate the genetic basis of a sex-linked meiotic driver in the Malaysian stalk-eyed fly, Teleopsis dalmanni. These data are generated from wild adult flies from the Gombak District of Peninsular Malaysia. 
, , , # Single-cell RNA sequencing of the testis of the drive and standard stalk-eyed fly.

[https://doi.org/10.5061/dryad.zkh1893kb](https://doi.org/10.5061/dryad.zkh1893kb)

## Description of the data and file structure

**Samples**

Flies were reared at University College London from a wild-caught population originating from the Gombak Valley, Malaysia. All flies and larvae were incubated and reared at 25°C and fed on a diet of sweetcorn. To ensure known genotypes of samples, a homozygous drive (SR) population is maintained through a series of crosses as described in [(Presgraves et al., 1997)](https://paperpile.com/c/UqA1L6/3Hym). Eight adult males, four standard (ST) and four with drive (SR), were sacrificed before the dissection of both testes in iced phosphate-buffered saline (PBS). These adults were all virgins, reproductively mature, and reared from egg lays collected on the same day. 

***Tissue collection, dissociation, and single-cell sequencing***

Testis pairs were individually ...,