Identification of Candidate Biomarker Proteins Released by Human Endometrial and Cervical Cancer Cells Using Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry

Candidate biomarker proteins, including chaperonin 10 and pyruvate kinase, previously discovered
and identified using mass-tagging reagents with multidimensional liquid chromatography and tandem
mass spectrometry (DeSouza, L.; et al. J. Proteome Res. 2005, 4, 377−386) have been identified in
serum-free media of cultured endometrial cancer (KLE and HEC-1-A) and cervical cancer (HeLa) cells.
These and other cancer-associated proteins were released by the cultured cells within 24 h of growth.
A total of 203 proteins from the KLE cells, 86 from HEC-1-A, and 161 from HeLa are reported.
Keywords: Endometrial cancer • Cell released proteins • Candidate biomarkers • Two-dimensional liquid
chromatography/tandem mass spectrometry