Reciprocal crosses between Ospie1 hetrozygotes and wild-type.

Reciprocal crosses between wild-type and Ospie1-1 or Ospie1-2 heterozygous plants were performed and the setting rates for each cross were scored. Data represented the average ±SD for 5–8 plants in each cross. No statistical significant difference was observed in setting rates between these genotypes. Those filled grains in F1 were then PCR genotyped to determine the number of Ospie1 heterozygotes and wild-type. X2-test was used to determine that they followed the 1∶1 segregation ratio. A cross involving wild-type (WT) was also performed as a control.